Pathogensis Staphylococcus aurous

Pathogensis Staphylococcus aurous

Catalase test: 

Hydrogen peroxide break down to form oxygen (O₂₎and water (H₂O) with the help of catalase ,when an organism come into contact with hydrogen peroxide and release bubble of oxygen that indicates the indicates the organism is catalase producer.

H₂O₂ + Bacteria    Catalase    H₂O+O₂ ↑

Requirements:

1.Test tube

2.H₂O₂-3%

3.Glass rod

Procedure:

    1.Take 3ml of H₂O₂ solution in a test tube

    2.Using a glass rod, remove several colonies of test organisms.

    3.Then immerse the colonies into H₂O₂ solution .

    4.Look for immediate bubbling.

Result:

    1.Active bubbling-------------→Catalase positive.

    2.No bubbling-------------→Catalase negative

Pathogen:

Catalase positive: Staphylococcus aurous.